mesophilic starter cubes next to white bowl

How to Make Mesophilic Starter Culture for Cheesemaking?

How to Make Mesophilic Starter Culture for Cheesemaking

Among the ingredients in my traditional mozzarella cheese recipe is mesophilic culture cubes. Which I’m sure most folks don’t need and those of us who do, don’t have on hand. DIY Mesophilic starter culture is so easy to make it just seems silly to go buy comparatively expensive cultures!

Mesophilic starter culture is a low-temperature cultured product. Just like buttermilk and yogurt. It provides cheeses with bacterial organisms that assist in proper curd formation, taste, texture, and aroma. Of course, this means that if your starter culture is off-tasting, your cheese may be off-tasting as well. There is some security (and extra expense) in using prepared mesophilic starter cultures like this one. And different mesophilic starter cultures work best for different types of cheeses. (For example, the one I linked to is good for Cheddar, Jack, and Colby cheeses.)

Knowing how to make your own mesophilic starter culture is useful information. It helps you gain a better understanding of the cheese-making process. Also, it gives you a greater sense of “self-sufficiency” in the event that you can’t get access to prepared cultures. It’s wise to know how to do something the independent way first. Just to have the knowledge filed away.  Once you have learned the new skill set, take advantage of the conveniences available in our modern times. Then you can rest, assured that you’ll know what to do when and if “one day” ever comes.

How to Make Mesophilic Starter Culture for Cheesemaking

How to Make Mesophilic Starter Culture for Cheesemaking

  • Prepare Cultured Buttermilk.
  • Freeze in ice cube trays.
  • Save in a storage bag in the freezer until ready to use. One cube is the equivalent of one ounce of mesophilic culture.

Yep, it’s that easy!

Suggested Read: Essential Guide To Raw Milk (Plus 115 Recipes)

Final Thoughts

Now, the results with homemade mesophilic starter culture will not be as consistent as a manufactured mesophilic starter culture. But if you’re fine with subtle variations in the flavor of your cheese, then this is a perfect, frugal solution!

What cheese will you make next? You should try out this cheddar cheese recipe at home.

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10 Comments

    1. It’s the same process. Whether you’re simply aging it on the counter or adding a starter culture. Actually, if you make it before, you’re making cultured milk (which you could then make into butter and get cultured butter and cultured buttermilk.) We don’t like cultured butter here and make sweet cream butter instead. So I culture the buttermilk after butter making. Hope that explanation helped!

  1. I’m a bit confused. I have a gallon of whole raw milk. I bought some vegetables rennet. So if I buy a quart of cream and make butter, use the butter milk from that ? That has the culture already in it ? Sorry, I’m a bit dense. Lol

    1. Ok, so you can make butter 2 ways- with sweet cream or cultured cream. If you do the cultured cream, the buttermilk is already cultured. Go ahead and use it as your mesophilic starter. If you (like us) think cultured butter tastes funky and prefer to make sweet cream butter, then you culture the buttermilk after making butter. Does that make more sense?

  2. I have read several recipes on making your own mesophilic starter. Most of them have said to add a store-bought mesophilic starter, and boil the jar and, let sit out on the counter, etc.
    But you’re saying that it only takes three steps! Is that all? And it works for you?

  3. I am confused and I think I just did this wrong. :-] I let my milk sit out and then poured half a cup into Buttermilk from the store. It wasn’t until I look at the comments that I saw I was supposed to make butter 1st. So it this batch a dud? Can you clarify what repeat means. Sorry if I seem dense! Thanks for your help.

    1. NO! It’s not a dud! You could use your own buttermilk if you wanted to, but absolutely don’t have to! It’s basically about making a cultured milk product to culture your low temp cheese or other milk products that need a starter culture. I would totally use it! I’ve been away from my computer most of the day and didn’t see your comment until now- I hope you didn’t throw it out yet 🙂

  4. I was wondering how they made aged cheese prior to stores that sold the culture. Lol. Can you use kefir instead of cultured butter milk?

    1. I’ve never tried Wendy, but I don’t see why not. You’ll have to let us know how it works 🙂